To investigate the virological properties of a SARS-CoV-2 variant, Omicron BA.1, we generated chimeric recombinant viruses that express GFP and encodes a variety of S genes. To verify the genome seque
The PRV str. Becker genome (GenBank JF797219.1) was cloned as a BAC using the pBLueLox plasmid generating the BAC pBecker2 that produces the vBecker2 virus by transfection. In this way were build BAC9
Gene expression of nonsegmented negative-sense RNA viruses involves sequential synthesis of monocistronic mRNAs and transcriptional attenuation at gene borders resulting in a transcript gradient. To a
