Opposite-polarity motors activate one another to trigger cargo transport in live cells

The time-lapse series is taken from a Drosophila S2 cell plated on a Concanavalin A coated substrate in the presence of 1uM Cytochalasin D. Kinesin heavy chain has been previously depleted by RNAi. GFP-tagged peroxisomes GFP-tagged peroxisomes do not move in microtubule-filled processes. Use Figure 1B (top panel) as a marker for location of processes and cell body. Images were acquired every 1s for a period of 2 minutes.