Dietary induced epigenetic reprogram in mouse intestinal enterocytes

Bl6 mice were fed with New Western diet 1 (NWD1) or Americal Instititue of Nutrition 76A (AIN76A or AIN) diet from weaning for 3 months. Single intestinal epithelial cells were FACS isolated by the Epcam+, CD45- , and subject to scACTAseq assay. Examine roles of dedifferentiation of Bmi1 cell in regeneration of intestinal stem cells and its impact on mucosal homeostasis under normal physiogical condition (fed with various diets).