Complex cell-state changes revealed by single cell RNA sequencing of 76,149 microglia throughout the mouse lifespan and in the injured brain

Single cell RNA sequencing of FACS purified mouse microglia from embryogenesis to old age, and following injury using a demyelinating mouse model. 41 total animals, 3-4 replicates per timepoint and condition. E14.5, P4/P5, P30, P100, P540, and Injury本数据集为经荧光激活细胞分选术（Fluorescence-Activated Cell Sorting, FACS）纯化的小鼠小胶质细胞的单细胞RNA测序数据，样本覆盖胚胎发育至老年的多个生理阶段，并包含采用脱髓鞘小鼠模型构建的损伤后样本。本研究共使用41只实验动物，每个时间点与实验条件均设置3-4次生物学重复。涉及的时间点与分组包括：胚胎发育第14.5天（E14.5）、出生后第4/5天（P4/P5）、出生后第30天（P30）、出生后第100天（P100）、出生后第540天（P540）以及损伤组。