Autophagy in gastrocnemius is inhibited in Glucose-infusion hyperglycemia rats (GLU-rats).
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A: GLU-rats were treated with 50% glucose via carotid artery infusion for 24 h (2 mg/kg/h). Control rats were treated with an equivalent volume of 0.9% sodium chloride for 24 h. Blood glucose and insulin levels were measured before the rats were killed. B: mRNA levels of LC3, Atg5, Atg7, Atg12, BECN1 were detected by RT-PCR in gastrocnemius. C: Gastrocnemius lysates were analyzed by Western blot using anti-LC3, anti-Beclin 1, anti-ATG7 and anti-Actin. (*P
创建时间：
2016-02-29
