USP14 is a cysteine protease deubiquitinase associated with the proteasome and plays important catalytic and allosteric roles in proteasomal degradation. USP14 inhibition has been considered a therape
moTAP-ANKIB1 was purified from transduced HeLa by using M2 beads followed by streptavidin beads. Once purified, in vitro ubiquitin assay was performed in cell free assay during 1h at 37°C. Purified A