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Identification of the ubiquitin linkages generated by ANKIB1

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NIAID Data Ecosystem2026-05-10 收录
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moTAP-ANKIB1 was purified from transduced HeLa by using M2 beads followed by streptavidin beads. Once purified, in vitro ubiquitin assay was performed in cell free assay during 1h at 37°C. Purified ANKIB1 was incubated with or without UBE1, UBE2D3, ATP regeneration solution and ubiquitin molecules.

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2026-03-03
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