Transcript RNA collected from microglia cultured in the presence of B. burgdorferi (Bb) was compared to that of RNA collected from microglia (μG) cultured in medium alone. RT-PCR reactions were run in
SE = standard error.Log2 fold changes of the four specifically amplified genes for validation resulting from the MACE analysis and RT-qPCR (calculated using REST with correction of amplification effic
Values for qPCR results represent means of three biological replicates. *Contigs were originally classified based on 454 sequencing results. The tendency (nodule-enhanced or root-enhanced expression w