Sequencing of dengue virus PCR amplicons using the Illumina and Oxford Nanopore MinION platforms
收藏NIAID Data Ecosystem2026-03-12 收录
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We evaluated a method designed to address this challenge, using the 'Primal Scheme' multiplex PCR tiling approach to rapidly generate short, overlapping amplicons covering the complete DENV coding-region, and sequencing the amplicons on the portable Nanopore MinION device. The resulting sequence data was assessed in terms of genome coverage, consensus sequence accuracy and by phylogenetic analysis.
创建时间：
2020-12-30
