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GAS1 is required for Notch-dependent facilitation of SHH signalling in forebrain development

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A Gas1 knockout was made by replacing the Gas1 coding region with a LacZ gene. The ventral neuroepithelial midline of the rostral diencephalon of Gas1 wild type and mutant embryos at E10 were isolated using laser capture microdissection. In total five replicates for wild type and four replicates for Gas1 mutant embryos were analyzed. Each replicate consisted of a pool of five embryos of the respective genotype.

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