Titrating Avidity of Yeast-Displayed Proteins Using a Transcriptional Regulator

Data underlying the figures in the publication: Lopez-Morales, J. <em>et al.</em> “Titrating Avidity of Yeast-Displayed Proteins Using a Transcriptional Regulator” <em>ACS Synth. Biol.</em> <strong>2023</strong>, <em>12</em>, 419-31, https://doi.org/10.1021/acssynbio.2c00351 <strong>Table of Contents</strong> <strong>Fig1_subplots_EMpty pJL100 full atc range analysis.xlsx:</strong> Flow cytometry data of displaying fraction and median fluorescence for Fig. 1c and d. <strong>Fig2_subplots_20.08.05 tit disp GOx ABTS.xlsx:</strong> Flow cytometry data of displaying fraction and median fluorescence of Gox variants; GOX enzymatic activity assay data for Fig. 2 <strong>Fig3_subplots_ SDA data for sharing.xlsx and disks + sup data plot.pptx:</strong> Spinning disk assay data and micrographs for Fig. 3. <strong>Fig4_ subplots_20220421 YTD affibody aTc vs Gal titration curves.xlsx and 20220423 Gal good volume.xlsx: </strong>Flow cytometry data and PDL1 titration on yeast data for Fig. 4. <strong>Supplementary_Gal comparison_</strong> <strong>Gal time point-2.xlsx: </strong>Flow cytometry data for comparing the YTD system to the standard yeast surface display for sup. Fig. S1. <strong>Supplementary_Fig S3_Titrated Affibody Kd analysis.xlsx and Simulations.pzf:</strong> ICE tables and simulation spreadsheet for sup. Fig. S3.