Arterial Sca1+ Vascular Stem Cells Generate De Novo Smooth Muscle for Artery Repair and Regeneration

Mice were bred with normal diet and maintained on a C57BL6/ICR background.For treatment, tamoxifen dissolved in corn oil (20 mg/ml) was introduced by gavage at the indicated time.All mice were randomly assigned to groups (including both male and female), and the investigators who analyzed the samples were blinded to the group allocations.We obtained staining images by microscope, single cell data by sequencing, and related flow data by flow analysis and sorting. The image results were analyzed by Image J and plotted by prism.