Microbial community analysis via high-throughput sequencing of amplified 16S rRNA genes is an essential microbiology tool. We found the popular primer pair 515F (515F-C) and 806R greatly underestimate

NIAID Data Ecosystem30

microbial community diversties of aging flue-cured tobaccos

烟草陈化微生物群落

细菌多样性

Species diversity of surface bacterial communities on aging flue-cured tobaccos

NIAID Data Ecosystem20

Summary of the amplification range and discriminatory power predicted for the mammalian 16S rRNA primers.

16SrRNA引物评估

条形码物种鉴定

The table indicates, for each mammalian order, the number of species deposited in NCBI for the 16S rRNA genes, the number of species predicted to be amplified by the universal primers as well as the p

NIAID Data Ecosystem30

Additional file 1 of PrimerEvalPy: a tool for in-silico evaluation of primers for targeting the microbiome

微生物组学

16SrRNA引物评估

Additional file 1: Oral-bacteria database of the 16S rRNA gene sequences which was used for the coverage analysis.

NIAID Data Ecosystem20