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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and NELL-1(+/6R) Mouse Hippocampus Transcriptomes

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The goal of this study is to profile and compare the hippocampus transcriptomes of wild type (WT) and NELL-1(+/6R) mice to gain insight on their neurological function. Hippocampus tissues were isolated from 3-month-old adult WT and NELL-1(+/6R) mice. Total RNA was isolated using Qiagen Rneasy Lipd Tissue Mini Kit. NGS was conducted on Illumina HiSeq3000 as single read at 1 x 50 read length.

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