scRNAseq of FACS-isolated microglia from spinal cords of Optn KO mice
收藏Alliance of Genome Resources2026-08-01 收录
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CD11b+ CX3CR1+ CD45-Int microglia were FACSorted from Optn KO mice and single cell RNA sequenced using the SmartSeq2 protocol. SmartSeq2 single cell RNA sequencing
从Optn基因敲除小鼠中通过荧光激活细胞分选（Fluorescence-Activated Cell Sorting，FACS）分离得到CD11b阳性、CX3CR1阳性、CD45中间表达型的小胶质细胞（microglia），随后采用SmartSeq2测序方案开展单细胞RNA测序（single cell RNA sequencing）。SmartSeq2单细胞RNA测序
