Bioaroma generation: research studies on the sustainable enzyme-catalysed synthesis and advanced gas chromatography analysis of flavour and fragrance compounds

This interdisciplinary thesis explores the potential of enzyme-catalysed reactions as a more sustainable and eco-friendly alternative for the production of aroma compounds. Analytical methods based on gas chromatography were designed to assess the changes in each stage of the enzyme study, including characterisation, screening, optimisation and application to essential oil samples. Seven enzymes from two different classes were investigated. The best-performing catalyst reacted with 49 of the 72 alcohols identified amidst the samples’ complex background. The methods and findings of this research impacts the development of improved and green biocatalytic processes that can generate innovative and safer natural products.