Diversity of microbial community (bacteria, micro-eukaryotes and fungi) in the Passage of Lascaux Cave.

In the Passage of Lascaux Cave (France), the potential effect of season, mineral substrate and presence of stains was tested on present (DNA) and active (RNA) microbial community with high-throughput sequencing.Three genes markers of microbial diversity were analyzed, the 16S rRNA genes specific for bacteria, the 18S rRNA genes specific for micro-eukaryotes and the second internal transcribed spacer (ITS2) specific for fungi. Sequences corresponding to active microbial community were named with c.