DNA methylation profiles of 4T1 invasively-distinct subpopulations. DNA methylation profiles of 4T1 invasively-distinct subpopulations

Solid tumors exhibit significant phenotypic heterogeneity. This phenotypic heterogeneity yields the formation of phenotypically distinct subpopulations of cells within a single tumor. Within the 4T1 murine mammary adenocarcinoma cell line, we observe cells that invade either in collective chains or as single cells. Through this study, we sought to dissect the epigenetic differences between these invasively-distinct subpopulations. Overall design: Five total groups were assessed, each in triplicate (15 samples total). No controls or reference groups were included, and the data for each group was compared to each other group.