活细胞长时程结构光超分辨数据集

项目课题1使用自主搭建的GI-SIM显微镜技术观测细胞中微管的荧光图像，数据采集于2017年9月。数据采集并完成超分辨重建。图像分析软件image-J分析了微管图像的半高全宽（即空间分辨率）。课题1课题组使用自主搭建的ATFM显微镜技术观测单层荧光bead图像的像散图像结果，数据采集于2018年4月。这个数据是用于单张荧光图像获得荧光微球的三维位置信息。图像分析软件image-J制作成时间堆栈的TIF数据。Project 1 utilized a self-assembled GI-SIM microscopy system to capture fluorescence images of intracellular microtubules. The data was collected in September 2017, and post-acquisition super-resolution reconstruction was performed. The full width at half maximum (FWHM, i.e., spatial resolution) of the microtubule images was analyzed using Image-J software. Project 1’s team employed a self-built ATFM microscopy platform to obtain astigmatic images of single-layer fluorescent bead samples. The data was collected in April 2018, and this dataset is designed to extract three-dimensional positional information of fluorescent microspheres from a single fluorescence image. All related data was processed into time-stack TIF format data using Image-J software.

搜集汇总

背景与挑战

背景概述

该数据集包含使用自主搭建的GI-SIM和ATFM显微镜技术采集的活细胞长时程结构光超分辨图像，主要观测细胞微管和荧光bead，数据采集于2017年9月和2018年4月。数据集涉及超分辨重建和空间分辨率分析，总数据量为118.28MB，包含2个文件。