Aptamers are molecules that reveal highly complex and refined molecular recognition properties. These molecules are capable of binding with high affinity and selectivity to targets, ranging from small
We genearted a DNA aptamer against EPX via SELEX protocol using a random DNA library and developed an assay to monitor EPX in patients spitum samples After 15 rounds of selection, the aptamer sequence
The excel file contains modeled distributions of Kd values for progressive rounds of aptamer selection and proposed bulk affinity workflow. The origin file includes the construction of gaussi
Library-immobilized SELEX was performed with an N30 stem-loop structured DNA library to isolate an aptamer that binds to the small molecule flunixin. Pools from various trials were sequenced.
The primer and library sequences used in this study as well as the sequences of the two aptamers discovered in this work. APTSEB1 was reported in 48 out of 49 sequences.
