Global analysis of Arabidopsis thaliana DICER-LIKE1 RNA substrates

By introducing point mutations in the RNAse III domains of plant DCL1 we generated Catalytically inactive (ci) DCL1. Plant expressing DCL1ci show an increase stability of the RNA/DICER complexes and therefore represent an innovative strategy to perform a direct and systematic analysis of DCL1 substrates. Overall design: Triplicates of pDCL1-DCL1ci-HA/dcl1-7 and respective dcl1-7 control plants RIP-seq