Best gene deletion strategies identified by OptORF using our MIP techniques for acetate production under glucose anaerobic condition.

a‘Changes’ refer to the number of genes which are newly introduced in the solution with k deletions or removed from the solution with k–1 deletions. bYield is reported as % of the TMP for wildtype strain with a maximum glucose uptake rate of 10 mmol gDW−1 h−1 (2.56 mol acetate produced/mol glucose consumed). gDW stands for gram dry weight.