All operations were carried out at 4°C. Cell-free extract and (NH4)2SO4 precipitation: 1 U = reduction of 1 µmol 4′-chloroacetophenone per min. DEAE Sepharose and Native-PAGE: 1 U = oxidation of 1 µ
Background: Knowing chromatin components at a DNA regulatory element at any given time is essential for understanding how the element works during cellular proliferation, differentiation and developme
