Neurog3-Cre p300/CBP mouse islets RNA-seq

We have performed RNA-seq on mouse islets lacking all p300 (p300 KO), all CBP (CBP-null), and one copy of CBP and all p300 (triallelic). The data revealed that p300 and CBP regulate some distinct but largely overlapping genes in islets. This was further confirmed by GO term and transcription factor target analyses, which suggested that these coactivators regulate genes that function similarly and converge to Hnf1a pathway. Six WT, three p300IsletKO, three CBPIsletKO and three CBPHet; p300KO samples were sequenced in two batches at UBC Biomedical Research Centre Sequencing Core. Each library was sequenced to a depth of at least 20 million paired end reads on a NextSeq 500 (Illumina, CA, USA).