Genes identified by poly(A)+ transcriptome sequencing as overexpressed in trf5-Δ98-117

Purpose: To study the effects of disrupting the Trf5-Mtr4 interaction Methods: Analyzed TRF5 and trf5-Δ98-117 strains by transcriptome sequencing of poly(A)+ RNA Results: The set of most significantly affected genes included 71 that were overexpressed in trf5-Δ98-117 and only 5 that were down-regulated.The set of 71 overexpressed genes was predominated by known TRAMP substrates Conclusions: Disruption of the Trf5-Mtr4 interaction results in a snoRNA processing defect研究目的：探究破坏Trf5-Mtr4相互作用所产生的生物学效应。 研究方法：通过对聚腺苷酸化RNA（poly(A)+ RNA）进行转录组测序，分析TRF5菌株与trf5-Δ98-117菌株的基因表达情况。 研究结果：受影响最为显著的基因集合包含71个在trf5-Δ98-117菌株中呈过表达状态的基因，仅5个基因出现下调；该71个过表达基因主要由已知的TRAMP复合物底物（TRAMP substrates）构成。 研究结论：破坏Trf5-Mtr4相互作用会导致小核仁RNA（snoRNA）加工缺陷。