Molecular analysis in imiquimod (IMQ)-induced psoriasis model

The mechanisms underlying pruritus of imiquimod (IMQ)-induced psoriasis remain poorly understood. In this study, we investigated whether there are certain key signaling molecules downstream of the recently identified peptides mediating itch in the spinal cord. We investigated the changes in the trascriptome by performing RNAseq of cervical spinal cords in control and IMQ-induced psoriasis. Our study represents the detailed analysis of transcriptomes of spinal cord in chronic itch model with biologic replicates, generated by RNA-seq technology. Overall design: six mice (3 controls and 3 psoriasis) were sacrificed. Cervical spinal cord isolation is followed by RNA extraction of single samples, library preparation and sequencing.