Transcriptional profiling of Slc7a7-deficient bone marrow derived macrophages subjected to in vitro LDL loading

Atherosclerotic coronary artery disease (CAD) development encompasses changes in immune cell populations, including macrophages. In atherosclerotic plaques, macrophages engulf LDL, becoming lipid-laden foam cells. Human and mouse atherosclerosis single-cell RNA-Seq datasets revealed Slc7a7 as a gene expressed specifically in macrophages, particularly in the lipid-associated macrophage subpopulation. Here, we investigate the gene expression profiles of Slc7a7-deficient (Slc7a7-/-) and Slc7a7+/+ bone marrow-derived macrophages exposed to either acetylated or oxidized LDL in vitro. Overall design: Bulk RNA-Seq of Slc7a7 knockout or control mouse bone marrow-derived macrophages (BMDM) treated with acetylated LDL (AcLDL) or oxidized LDL (OxLDL).