M6A immunoprecipitation from 15min BrU labeled and fragmented nascent RNA. HEK293T cells were incubated with 2 mM bromouridine for 15 min labeling time (pulse). Whole-cell total RNA was extracted with
Table S3. Gene-centric tagging determined by iSNPs and a read-based method for the Fibroblast, Lymphoblast and Pool100 datasets on ChrX, Chr17 and all autosomal chromosomes. (XLSX 2939 kb)
Additional file 3: The m6A-peaks detected throughout this study in the yeast strains (Table S3), human-mice interspecies hybrid system (Table S4) and mice-mice intraspecies hybrid system (Table S5).
X-chromosome inactivation (XCI) is a global silencing mechanism by which XX and XY mammals equalize X-linked gene dosages. XCI begins with an establishment phase during which Xist RNA spreads and indu