We aimed to design a low-density genotyping platform using the genotyping by target sequencing (GBTS) technology. The markers of the new-developed GBTS platform were selected from diverse resources in
454 GS-FLX sequencing was used to identify the candidate genes involved in drought tolerance and to develop more EST-SSR markers in Z. nummularia. Roche GS-FLX454 pyrosequencing of drought stressed Z.
