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Single Mismatch CRISPRi pooled growth sequencing samples

NIAID Data Ecosystem2026-04-25 收录

CRISPRi筛选

竞争性生长实验

数据链接：

https://www.ncbi.nlm.nih.gov/sra/SRP223395 数据链接链接失效反馈

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资源简介：

Raw FASTQ data for [Hawkins JS, Silvis MR, et. al. 2020]. Samples reflect amplified identifier sequences from B. subtilis and E. coli strain collections before and after competitive growth experiments.

应用场景：

创建时间：

2020-10-13

单细胞转录组学

CRISPRi筛选

Methods: We create a CRISPR vector containing a polyadenylated RNA barcode and couple it with droplet scRNA-seq to get a large scale transcriptional measurements of perturbations Results: We were able

DataCite Commons2020-10-10 更新60

CRISPR screen identifies loss of IFNγR signaling and downstream adhesion as a resistance mechanism to CAR T-cell cytotoxicity in solid tumors

CART细胞抵抗机制

CRISPRi筛选

U87-CBG-GFP cells selected with blasticidin to stably express Cas9 were transduced with an MOI (< 1) of Brunello guide library (with puromycin resistance in the guide backbone). Cells were rested for

NIAID Data Ecosystem20

Targeting SUMO2 reverses aberrant epigenetic rewiring driven by SS18::SSX fusion oncoproteins and impairs sarcomagenesis [CRISPR Screening]

滑膜肉瘤治疗

CRISPRi筛选

Synovial sarcoma (SySa) is an aggressive soft tissue sarcoma with an urgent need to develop targeted therapies. Here, we exploited specific vulnerabilities created by transcriptional rewiring by the f

NIAID Data Ecosystem10

Additional file 16 of Identification of functional regulatory elements in the human genome using pooled CRISPR screens

CRISPRi筛选

功能基因组学

Additional file 16: Table S11. Guide-level MAGeCK output (Peak Library/Cas9/Untreated).

NIAID Data Ecosystem50

Parallel characterization of cis-Regulatory Elements for Multiple Genes using CRISPRpath [Cut&Tag]

CRISPRi筛选

植物顺式调控元件分析

Here, we describe CRISPRpath, a CRISPR screening strategy for simultaneously characterizing CREs in multiple genomic loci by leveraging converging phenotypes resulted from transcriptional defects in g

NIAID Data Ecosystem10

Single Mismatch CRISPRi pooled growth sequencing samples

资源简介：

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