Olfactory bulb gene expression profiling upon loss of Sox9 in adult astrocytes

The goal of this study is to determine how the loss of the transcription factor Sox9 affects the molecular profiles of adult astrocytes from the olfactory. We performed RNA-sequencing on wildtype and Sox9 knockout (KO) astrocytes from the olfactory bulb and analyzed the molecular signatures of Sox9 KO astrocytes compared to the wildtype control. AAV-GFAP-iCre-P2a-TurboRFP was injected into the olfactory bulbs of Sox9fl/fl mice or C57BL/6J mice to generate astrocyte-specific Sox9 knockout and control mice. FAC sorted astrocytes from the olfactory bulbs were collected in triplicate from our viral Sox9 KO mouse line and control mice 4 weeks after the viral injection. Sox9 KO and control astrocytes were molecularly profiled using RNA-sequencing.