Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [breaktag 1]

Here we developed BreakTag, a versatile, highly parallel and scissile-aware methodology for the profiling of Cas9-induced DNA double strand break (DSBs), to identify molecular determinants influencing Cas9 incisions Overall design: Map the off-target landscape and characterization of the scission profile of 1491 genomic gRNA-targets (HiPlex1 library) coupled with LZ3 using BreakTag