Randomized PAM depeletion of NmeCas9
收藏NIAID Data Ecosystem2026-05-26 收录
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资源简介：
We report the PAMs of NmeCas9 using a cell-free TXTL-based cleavage assay. By adding randomized PAM library and NmeCas9-gRNA in vitro, functional PAM sequences were cleaved, while non-functional PAMs remained. By amplifying the non-cleaved DNA, we use next-generation sequencing to analyze the depletion of functional PAMs of NmeCas9. Overall design: Pam determination assay for NmeCas9
创建时间：
2019-02-23
