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Transcriptomic analysis of BNC2 regulated genes in mouse EMS404 cells

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NIAID Data Ecosystem2026-03-14 收录
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Mouse myofibroblastic EMS404 cells were transfected using a siRNA directed against BNC2 (siBNC2) or a control siRNA (siCTRL) and RNA were processed for microarray analyses. Transfection of siRNA (used at 20 nM) into EMS404 cells was performed using lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). siBNC2 (Cat# 4390771 s109765) and Silencer select negative control (Thermo Fisher Scientific Cat# 4390844) were used. 48h after transfection, RNA was purified and processed for analysis using Affymetrix GeneChip arrays (MoGene2.0st) according to standard procedures.

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2022-09-27
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