Dissociation constants (K<sub>d</sub>) in mol/L and associated errors of CASTing targets.
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Relative binding affinities of the CASTing targets to AtMYB61 were determined by a nitrocellulose filter-binding assay. The relative binding affinities were used to determine the dissociation constants of the CASTing targets by GRAFIT program which linearised the nonlinear regression via scatchard plots to calculate the point at which half of the ligand was bound to AtMYB61. ACCTAC bound with the greatest affinity to AtMYB61. NBS or non-binding site did not bind to recombinant AtMYB61.
创建时间：
2013-05-31
