Kd values for RTs on various duplex constructs.

1Refer to Fig. 1 for duplex sequences.2Experiments for determining Kd values are illustrated in Fig. 2. All values are an average of 2–4 independent experiments ± standard deviations. Values are in nM and were calculated based on protein mass as provided by the manufacturer (HIV, MuLV, and AMV RTs) or determined as described (Ty3) [31]. Active site concentrations were not determined.1. 双链序列详见图1。 2. 解离常数（Kd）的测定实验详见图2。所有数值均为2至4次独立实验的平均值，以±标准偏差表示误差范围。数值单位为纳摩尔（nM），其中针对人类免疫缺陷病毒（HIV）、鼠白血病病毒（MuLV）以及禽成髓细胞瘤病毒（AMV）的逆转录酶（RTs），其数值基于厂商提供的蛋白质量计算得到；针对Ty3逆转录酶，则按照文献[31]所述方法测定。未测定活性位点浓度。