We modified a long-range amplicon sequencing method with high accuracy to determine CRISPR-induced large deletions by developing a novel k-mer sequence alignment algorithm. We observed that the large
We measured global RNA levels using RNA-seq in Δcas3 E. coli cells with intact CRISPR arrays or cells with the CRISPR-I array deleted to determine if off-target events driven by endogenous spacers aff
