The role of ALKBH5 in maternal RNA decay during mammalian oocyte meiosis

To investigate the function of ALKBH5 in the regulation of maternal RNA decay during mice oocyte meiosis, we established Alkbh5 knockout mice and analyzed the m6A level changes between control and Alkbh5 knockout oocyte at GV stage Comparative m6A peaks analysis of RNA-seq data for control and Alkbh5 knockout oocytes at GV stage为探究ALKBH5在小鼠卵母细胞减数分裂过程中调控母源RNA降解的功能，本研究构建了Alkbh5基因敲除小鼠，并分析了生发泡期（Germinal Vesicle, GV）对照与Alkbh5敲除卵母细胞的m6A（N6-甲基腺苷）水平变化；同时针对GV期对照及Alkbh5敲除卵母细胞的RNA测序（RNA-seq）数据开展了对比m6A峰分析。