A variety of methods have been developed for the repression of gene expression using CRISPR-Cas systems, where a catalytically dead Cas protein (dCas) is used to regulate genes in an RNA-guided manner
To investigate the effectiveness of CRISPR based gene modulation, IRF7 was repressed in dCas9-KRAB DF1 cell line or activated in dCas9-VPR DF1 cell line followed by whole transcriptome analysis Overal
This file contains the microscopy images of the data in Fig. S9 and S10 for the following condition: 2 hours of CRISPRi induction (43% induction level) followed by inducers removal and the addition of
Transcriptional enhancers orchestrate cell-type specific gene expression programs critical to eukaryotic development, physiology, and disease. However, despite the large number of enhancers now identi