Expression data of nuclear mRNA export mutant rae1-167 cells. Schizosaccharomyces pombe

rae1 is an essential gene and encodes one of nuclear pore complex. rae1-167 mutant cells show rapid accumulation of polyA-RNA in the nucleus at 36°C followed by protein accumulation, suggesting that accumulated nuclear mRNA influences nucelar cytooplasmic transport. We used microarrays to compare the overall gene expression of rae1-167 cells grown at 36°C with that at 25°C. Overall design: rae1-167 cells grown in YE4S medium were harvested for RNA extraction and hybridization on Affymetrix microarray. In order to characterize transcriptional profiling of rae1-167 cells at 36°C, we carried out the genome-wide gene expression analysis of rae1-167 cells.