m6A MeRIP-seq was performed to investigate the divergent m6A modification patterns among different varieties of Oryza sativa at the transcriptome level.
M6A immunoprecipitation from 15min BrU labeled and fragmented nascent RNA. HEK293T cells were incubated with 2 mM bromouridine for 15 min labeling time (pulse). Whole-cell total RNA was extracted with
N6-methyladenosine (m6A) RNA methylation is the most abundant internal chemical modifications in eukaryotic messenger RNA (mRNA) as well as long non-coding RNA (lncRNA). Recently, m6A RNA methylation
N6-methyladenosine (m6A) methyltransferase METTL3 mainly mediates mRNA m6A methylation and plays important roles in various biological processes. Here we report a chromatin-based role for METTL3 in he