Additional supplementary data for the paper. ClusterLogos.pdf - The logo sequences for each cluster of the gRNAs from LOC440792 gene. gRNA.xlsx -The set of gRNAs found in Chromosome 22 of HG38 human r
To inverstigate off-target effects of CRISPR-iPAS, we transfected HEK293TAPA reporter cells with dPguCas13b and gUSE (showed most effective APA-interference) or non-targeting gRNA (gNT) respectively.
The APC, KRAS, P53 and SMAD4 genes were mutated in human small intestinal stem cells and colon stem cells, which grow in vitro as epithelial organoids. Here we assessed candidate off-target sites for