Primers for LIC-based assembly.

PCR primers used for the assembly of palindromic RNAi constructs The first round PCR sequences correspond to the 5′ tags with are tethered to the gene specific primer sequences The second round PCR primers contain dU residues used to generate ss tails The vector overhang depicts the single stranded regions of the vector which are generated following HpaI and NbBbvCI digestion The left and right ss vector overhangs are complementary to the 3′ tails generated by UDG cleavage of dUa5′ and dUs3′ of their respective PCR products.