Next Generation Sequencing Facilitates Quantitative Analysis of Brown adipocytes transcriptomes from Control (LrpprcFF) and LrpprcBKO (LrpprcFF, Ucp1-CRE) mice

Next-generation sequencing (NGS) brings us the opportunity to analyze cellular pathways. The main goal of this study was to compare brown adipocyte transcriptome profiling (RNA-seq) between Control and LrpprcBKO mice housed at room temperature or at thermoneutrality. Brown adipocytes mRNA profiles of 8-week old wild type (WT) and LrpprcBKO mice were generated by deep sequencing, in triplicate or quadruplicate, using Illumina HiSeq 2000, by Novogene.