Simultaneous profiling of transcriptome and chromatin accessibility in single nucleus

Here we describe SNARE-seq, a droplet-based method to simultaneously profile gene expression and chromatin accessibility in each of thousands of single nuclei. Employing this method, we profiled 1047 nuclei from human cell line mixture, 5081 and 10309 nuclei from neonatal and adult mouse brain. Single nuclei RNA-seq and ATAC-seq co-assay for BJ, H1, K562 and GM12878 cell mixture, and wild type neonatal and adult mouse brain cerebral cortex.本文介绍SNARE-seq（SNARE-seq）：一种基于微滴的单细胞核多组学检测技术，可在数千个单细胞核中同时实现基因表达与染色质可及性的并行检测。本研究依托该技术，共完成三类样本的单细胞核检测：人类细胞系混合样本中的1047个单细胞核，新生小鼠脑组织中的5081个单细胞核，以及成年小鼠脑组织中的10309个单细胞核。本次检测涵盖BJ、H1、K562及GM12878细胞系混合样本，以及野生型新生小鼠与成年小鼠大脑皮层的单细胞核RNA测序（RNA-seq）与转座酶可及性染色质测序（ATAC-seq）联合检测数据。