Gene redundancy complicates systematic characterization of gene function as single-gene deletions may not produce discernible phenotypes. Dual-TnSeq (dual transposon sequencing) couples random barcode
tRNAs decoding standard 20 AA 58 Selenocysteine tRNAs (TCA) 0 Possible suppressor tRNAs (CTA,TTA,TCA) 0 tRNAs with undetermined or unknown isotypes 1 Predicted pseudogenes 0 Total tRNAs 59
Gene redundancy complicates systematic characterization of gene function as single-gene deletions may not produce discernible phenotypes. Dual-TnSeq (dual transposon sequencing) couples random barco