Dual randomly barcoded transposon sequencing (Dual Tn-seq) data for <em>Streptococcus pneumoniae</em> D39

Gene redundancy complicates systematic characterization of gene function
as single-gene deletions may not produce discernible phenotypes.
Dual-TnSeq (dual transposon sequencing) couples random barcode transposon
site sequencing with the Cre-lox system to enable the characterization of
&gt;1 billion double mutant strains. This data set reports dual-tnseq
data for Streptococcus pneumoniae D39 in rich media. Large libraries of
mutants with two different transposons, carrying two different resistance
markers and each with their own barcodes, were constructed and sequenced.
Then, two libraries of mutants were combined by using transformation and
selected on rich media; the Cre-lox system was induced to place the two
barcodes into proximity; and the pairs of barcodes were sequenced. Pairs
of genes with low rates of dual insertions often indicate synthetic
lethality or a milder genetic interaction. The genetic interactions
identified span a wide range of biochemical processes and reveal new
factors in well-studied pathways, including a novel cytidine triphosphate
synthase and an activator of cell wall biosynthesis.