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Sort-seq assays of CggR-cpGFP biosensor libraries

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NIAID Data Ecosystem2026-03-13 收录
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Libraries of single fluorescent protein biosensor variants were constructed with variation in either the site of GFP insertion into the CggR ligand-binding domain or the composition of amino acid linkers connecting domains. The pooled libraries were characterized by a massively parallel assay combining FACS and DNA sequencing. Libraries were screened in HEK 293T TetBxb1BFP "landing pad" cell line.

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2022-01-01
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