RNA-sequencing analysis reveals the different gene expression in male and female wild type and TECRL knockout mice.

Purpose: In previous study, we generated the TECRL knockout mice. The purpose of this study is to indentify the implication function of TECRL on cardiac myocytes in male and female TECRL knockout(TECRL-/-) mice compared with wild type(WT) mice. Methods: Total RNA profiles of 5 males WT mice and 5 males TECRL-/- mice, 3 females WT mice and 7 females TECRL-/- mice were generated by RNA sequencing. The first strand cDNA was synthesized using random hexamer primer and the second strand cDNA synthesis was performed using DNA Polymerase and RNase H. Results: 1249 genes were identified between TECRL-/- mice and WT mice through RNA-seq analysis. GO analysis and KEGG analysis revealed some pathways, especially lipid metabolism and Ca2+ handling were closedly related to TECRL deficiency. Comparation of transcriptome of heart from 8 weeks male and female wild type(WT) and TECRL-/- mice.研究目的：既往研究中，我们已构建TECRL敲除（TECRL knockout）小鼠。本研究旨在对比野生型（wild type, WT）小鼠与雌雄两性TECRL敲除（TECRL-/-）小鼠，明确TECRL在心肌细胞中的潜在功能。 研究方法：通过RNA测序（RNA sequencing）获取了5只雄性野生型小鼠、5只雄性TECRL敲除小鼠、3只雌性野生型小鼠及7只雌性TECRL敲除小鼠的总RNA转录组数据。实验采用随机六聚体引物合成第一链互补脱氧核糖核酸（complementary DNA, cDNA），并借助DNA聚合酶与核糖核酸酶H（RNase H）完成第二链cDNA的合成。 研究结果：通过RNA测序分析，在TECRL敲除小鼠与野生型小鼠之间鉴定出1249个差异表达基因。基因本体（Gene Ontology, GO）富集分析与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）通路富集分析结果显示，多条通路与TECRL缺失密切相关，其中脂质代谢与钙离子（Ca²+）调控通路尤为显著。本研究还对8周龄雌雄野生型及TECRL敲除小鼠的心脏转录组进行了对比分析。