Cardiac Sex Differences in the Transcriptome of C57 Male and Female Adult Hearts

Purpose: The goals of this study are to compare adult male and female C57 mouse hearts transcriptome profiling (RNA-seq) to conclude cardiac sex differences at the mRNA level. Methods: mRNA profiles of adult male and female C57 mouse hearts were generated by deep sequencing, n=4 for each sex, using Illumina HiSeq2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows-Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. Results: Using an optimized data analysis workflow, we mapped about 50 million sequence reads per sample to the mouse genome (build mm10) and identified 16,160 transcripts in the mouse hearts. Adult male and female C57 mouse hearts RNAseq研究目的：本研究旨在对比成年雄性与雌性C57小鼠心脏的转录组谱（RNA-seq），以阐明mRNA层面的心脏性别差异。 研究方法：采用Illumina HiSeq2500平台对成年雄性与雌性C57小鼠心脏的mRNA表达谱进行深度测序，每个性别组设置4个生物学重复（n=4）。对通过质量过滤的序列读段，采用两种方法在转录本异构体层面开展分析：一是Burrows-Wheeler比对工具（Burrows-Wheeler Aligner, BWA）结合方差分析（ANOVA），二是TopHat结合Cufflinks。 研究结果：通过优化的数据分析流程，我们将每个样本约5000万条序列读段比对至小鼠基因组（版本mm10），并在小鼠心脏中鉴定出16160个转录本。成年雄性与雌性C57小鼠心脏的RNA-seq